cloning site meaning in English
插入位点,克隆位点
克隆位点
Examples
- Then the amplified mtb8 . 4 and ms gene were subcloned into the unique nhe i and mlu i cloning sites of pci - neo expression vector
重组质粒pm 、 pms转染ps15细胞，进行稳定表达， g418筛选抗性克隆后，用rl ’ - pcr检测目的基因在mrna水平的表达。 - On the base of construction of pbi121vp7 , we constructed the fusion gene expression vector pbi121ctbvp7 by the same sigle cloning site ( ndei and xbai ) of vector puc19ctb and pbi121vp7
设计具有ndei和saci单限制性酶切位点的vp7基因引物，通过pcr扩增出vp7基因并经测序验证。 - According to csfv ' s e2 gene sequence published in genbank and the sequence of p . pastoris expression vector ppic9 ' s multiple cloning site ( mcs ) , a pair of primers were designed with oligo and primers . o softwares
将该基因片段先克隆到pmd18 - t载体上，并进行了酶切、 pcr鉴定和测序分析，阳性重组质粒命名为t - e2 。 - Construction of two vectors containing different plasmid original replicon this work constructed four resolution shuttle vectors , pbmb1205 , pbmb1205r , pbmb1206 and pbmb1206r . there are multiple clone sites between two copies of res sites
解离载体的构建和性能利用来源于不同质粒上的质粒复制起始区ori44和ori1030构建了4个解离载体。 - A fusion - trascriptional library was then constructed by inserting sau3ai - partially digested chromosomal dna from 7653r into bamhi cloning site of phn127 . a group of constitutive - expression fusions with different fluorescent strength were obtained
将7653r的总dna经sau3ai部分酶解并克隆到phn127上，获得的融合子库，从中筛选到gfp组成型表达的具有调控活性的dna片段。